Raman spectroscopic study on the excystation process in a single unicellular organism amoeba (Acanthamoeba polyphaga).
J Biomed Opt
; 20(5): 51042, 2015 May.
Article
in En
| MEDLINE
| ID: mdl-25928386
ABSTRACT
An in vivo Raman spectroscopic study of amoeba (Acanthamoeba polyphaga) is presented. The changes of the spectra during the amoeba cyst activation and excystation are analyzed. The spectra show the changes of the relative intensities of bands corresponding to protein, lipid, and carotenoid components during cyst activation. The presence of carotenoids in the amoeba is observed via characteristic Raman bands. These signals in the Raman spectra are intense in cysts but decrease in intensity with cyst activation and exhibit a correlation with the life cycle of amoeba. This work demonstrates the feasibility of using Raman spectroscopy for the detection of single amoeba microorganisms in vivo and for the analysis of the amoeba life activity. The information obtained may have implications for the estimation of epidemiological situations and for the diagnostics and prognosis of the development of amoebic inflammations.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Acanthamoeba
/
Carotenoids
/
Protozoan Proteins
/
Lipid Metabolism
/
Life Cycle Stages
Type of study:
Prognostic_studies
Language:
En
Journal:
J Biomed Opt
Journal subject:
ENGENHARIA BIOMEDICA
/
OFTALMOLOGIA
Year:
2015
Document type:
Article
Affiliation country:
Taiwan