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A Novel Method for Rearing Zebrafish by Using Freshwater Rotifers (Brachionus calyciflorus).
Aoyama, Yuta; Moriya, Natsumi; Tanaka, Shingo; Taniguchi, Tomoko; Hosokawa, Hiroshi; Maegawa, Shingo.
Affiliation
  • Aoyama Y; 1 Division of Cognitive and Information Sciences, Faculty of Integrated Human Studies, Kyoto University , Kyoto, Japan .
  • Moriya N; 1 Division of Cognitive and Information Sciences, Faculty of Integrated Human Studies, Kyoto University , Kyoto, Japan .
  • Tanaka S; 1 Division of Cognitive and Information Sciences, Faculty of Integrated Human Studies, Kyoto University , Kyoto, Japan .
  • Taniguchi T; 1 Division of Cognitive and Information Sciences, Faculty of Integrated Human Studies, Kyoto University , Kyoto, Japan .
  • Hosokawa H; 1 Division of Cognitive and Information Sciences, Faculty of Integrated Human Studies, Kyoto University , Kyoto, Japan .
  • Maegawa S; 2 Department of Intelligence Science and Technology, Graduate School of Informatics, Kyoto University , Kyoto, Japan .
Zebrafish ; 12(4): 288-95, 2015 Aug.
Article in En | MEDLINE | ID: mdl-25938499
ABSTRACT
The zebrafish (Danio rerio) has become a powerful model organism for studying developmental processes and genetic diseases. However, there remain several problems in previous rearing methods. In this study, we demonstrate a novel method for rearing zebrafish larvae by using a new first food, freshwater rotifers (Brachionus calyciflorus). Feeding experiments indicated that freshwater rotifers are suitable as the first food for newly hatched larval fish. In addition, we revisited and improved a feeding schedule from 5 to 40 days postfertilization. Our feeding method using freshwater rotifers accelerated larval growth. At 49 dpf, one pair out of 10 pairs successfully produced six fertilized eggs. At 56, 63, and 71 dpf, 6 out of the 10 pairs constantly produced normal embryos. Our method will improve the husbandry of the zebrafish.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Rotifera / Zebrafish / Aquaculture / Diet Type of study: Prognostic_studies Limits: Animals Language: En Journal: Zebrafish Journal subject: BIOLOGIA Year: 2015 Document type: Article Affiliation country: Japan
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Rotifera / Zebrafish / Aquaculture / Diet Type of study: Prognostic_studies Limits: Animals Language: En Journal: Zebrafish Journal subject: BIOLOGIA Year: 2015 Document type: Article Affiliation country: Japan