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Triplex real-time PCR--an improved method to detect a wide spectrum of mitochondrial DNA deletions in single cells.
Rygiel, Karolina A; Grady, John P; Taylor, Robert W; Tuppen, Helen A L; Turnbull, Doug M.
Affiliation
  • Rygiel KA; Wellcome Trust Centre for Mitochondrial Research and Newcastle University Centre for Ageing and Vitality, Institute of Neuroscience, Newcastle University, Newcastle upon Tyne, NE2 4HH, UK.
  • Grady JP; Wellcome Trust Centre for Mitochondrial Research and Newcastle University Centre for Ageing and Vitality, Institute of Neuroscience, Newcastle University, Newcastle upon Tyne, NE2 4HH, UK.
  • Taylor RW; Wellcome Trust Centre for Mitochondrial Research and Newcastle University Centre for Ageing and Vitality, Institute of Neuroscience, Newcastle University, Newcastle upon Tyne, NE2 4HH, UK.
  • Tuppen HA; Wellcome Trust Centre for Mitochondrial Research and Newcastle University Centre for Ageing and Vitality, Institute of Neuroscience, Newcastle University, Newcastle upon Tyne, NE2 4HH, UK.
  • Turnbull DM; Wellcome Trust Centre for Mitochondrial Research and Newcastle University Centre for Ageing and Vitality, Institute of Neuroscience, Newcastle University, Newcastle upon Tyne, NE2 4HH, UK.
Sci Rep ; 5: 9906, 2015 May 19.
Article in En | MEDLINE | ID: mdl-25989140

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: DNA, Mitochondrial / Gene Deletion / Single-Cell Analysis / Multiplex Polymerase Chain Reaction / Real-Time Polymerase Chain Reaction Type of study: Diagnostic_studies Limits: Humans Language: En Journal: Sci Rep Year: 2015 Document type: Article Affiliation country: United kingdom Country of publication: United kingdom

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: DNA, Mitochondrial / Gene Deletion / Single-Cell Analysis / Multiplex Polymerase Chain Reaction / Real-Time Polymerase Chain Reaction Type of study: Diagnostic_studies Limits: Humans Language: En Journal: Sci Rep Year: 2015 Document type: Article Affiliation country: United kingdom Country of publication: United kingdom