A new method for measuring osteoclast formation by electrical impedance.
J Pharmacol Sci
; 128(2): 87-91, 2015 Jun.
Article
in En
| MEDLINE
| ID: mdl-26032840
ABSTRACT
Osteoclasts are important target cells for osteoporosis treatment. Recently, a real-time cell analysis (RTCA) system was developed to observe cell morphology and adhesion; however, the use of RTCA to study osteoclastogenesis has not been reported. Here, we investigated whether osteoclast formation could be monitored in real-time using RTCA. The cell index determined via electrical impedance using RTCA, and the number of osteoclasts exhibited a significant positive correlation. RTCA was useful for determining the effect of (-)-epigallocatechin-3-gallate on the inhibition of bone resorption. We established a new method of measuring osteoclast formation in real-time using RTCA.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Osteoclasts
/
Computer Systems
/
Cytological Techniques
/
Electric Impedance
Limits:
Humans
Language:
En
Journal:
J Pharmacol Sci
Journal subject:
FARMACOLOGIA
Year:
2015
Document type:
Article
Affiliation country:
Japan