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A new method for measuring osteoclast formation by electrical impedance.
Emori, Haruka; Iwai, Shinichi; Ryu, Kakei; Amano, Hitoshi; Sambe, Takehiko; Kobayashi, Takahiro; Oguchi, Tatsunori; Ohura, Kiyoshi; Oguchi, Katsuji.
Affiliation
  • Emori H; Department of Pharmacology, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan; Department of Orthopaedic Surgery, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.
  • Iwai S; Department of Healthcare and Regulatory Sciences, Showa University School of Pharmacy, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.
  • Ryu K; Department of Pharmacology, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.
  • Amano H; Department of Pharmacology, Osaka Dental University, 8-1 Kuzuhahanazono-cho, Hirakata, Osaka 573-1121, Japan. Electronic address: amano@cc.osaka-dent.ac.jp.
  • Sambe T; Department of Pharmacology, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.
  • Kobayashi T; Department of Pharmacology, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.
  • Oguchi T; Department of Pharmacology, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.
  • Ohura K; Department of Pharmacology, Osaka Dental University, 8-1 Kuzuhahanazono-cho, Hirakata, Osaka 573-1121, Japan.
  • Oguchi K; Department of Pharmacology, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.
J Pharmacol Sci ; 128(2): 87-91, 2015 Jun.
Article in En | MEDLINE | ID: mdl-26032840
ABSTRACT
Osteoclasts are important target cells for osteoporosis treatment. Recently, a real-time cell analysis (RTCA) system was developed to observe cell morphology and adhesion; however, the use of RTCA to study osteoclastogenesis has not been reported. Here, we investigated whether osteoclast formation could be monitored in real-time using RTCA. The cell index determined via electrical impedance using RTCA, and the number of osteoclasts exhibited a significant positive correlation. RTCA was useful for determining the effect of (-)-epigallocatechin-3-gallate on the inhibition of bone resorption. We established a new method of measuring osteoclast formation in real-time using RTCA.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Osteoclasts / Computer Systems / Cytological Techniques / Electric Impedance Limits: Humans Language: En Journal: J Pharmacol Sci Journal subject: FARMACOLOGIA Year: 2015 Document type: Article Affiliation country: Japan
Fulltext
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Osteoclasts / Computer Systems / Cytological Techniques / Electric Impedance Limits: Humans Language: En Journal: J Pharmacol Sci Journal subject: FARMACOLOGIA Year: 2015 Document type: Article Affiliation country: Japan