Genetic characterization of bovine viral diarrhea virus strains in Beijing, China and innate immune responses of peripheral blood mononuclear cells in persistently infected dairy cattle.
J Vet Sci
; 16(4): 491-500, 2015.
Article
in En
| MEDLINE
| ID: mdl-26119170
To acquire epidemiological data on the bovine viral diarrhea virus (BVDV) and identify cattle persistently infected (PI) with this virus, 4,327 samples from Holstein dairy cows were screened over a four-year period in Beijing, China. Eighteen BVD viruses were isolated, 12 from PI cattle. Based on genetic analysis of their 5'-untranslated region (5'-UTR), the 18 isolates were assigned to subgenotype BVDV-1m, 1a, 1d, 1q, and 1b. To investigate the innate immune responses in the peripheral-blood mononuclear cells of PI cattle, the expression of Toll-like receptors (TLRs), RIG-I-like receptors, interferon-α (IFN-α), IFN-ß, myxovirus (influenza virus) resistance 1 (MX1), and interferon stimulatory gene 15 (ISG15) was assessed by qPCR. When compared with healthy cattle, the expression of TLR-7, IFN-α, and IFN-ß mRNA was downregulated, but the expression of MX1 and ISG-15 mRNA was upregulated in PI cattle. Immunoblotting analysis revealed that the expression of interferon regulatory factor 3 (IRF-3) and IRF-7 was lower in PI cattle than in healthy cattle. Thus, BVDV-1m and 1a are the predominant subgenotypes in the Beijing region, and the strains are highly divergent. Our findings also suggest that the TLR-7/IRF-7 signaling pathway plays a role in evasion of host restriction by BVDV.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Bovine Virus Diarrhea-Mucosal Disease
/
Gene Expression Regulation
/
Diarrhea Virus 1, Bovine Viral
/
Immunity, Innate
Type of study:
Prognostic_studies
Limits:
Animals
Country/Region as subject:
Asia
Language:
En
Journal:
J Vet Sci
Journal subject:
MEDICINA VETERINARIA
Year:
2015
Document type:
Article
Affiliation country:
China
Country of publication: