Time-Lapse Imaging of Glial-Axonal Interactions.
Curr Protoc Neurosci
; 72: 2.23.1-2.23.14, 2015 Jul 01.
Article
in En
| MEDLINE
| ID: mdl-26131661
ABSTRACT
In the central nervous system (CNS), myelin is formed by oligodendrocytes that are derived from precursor cells, known as oligodendrocyte precursor cells (OPCs). Successive stages of OPC interactions with the axons can be visualized in vitro and ex vivo using mixed neural cell cultures and pieces of intact spinal cord, respectively. OPCs and their differentiation can be imaged using cell-type-specific markers or green fluorescent protein (GFP) tags. This protocol describes methodology for generating these two systems for time-lapse imaging of dynamic cell interactions using fluorescent and 2-photon microscopy.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Axons
/
Central Nervous System
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Neuroglia
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Time-Lapse Imaging
Limits:
Animals
Language:
En
Journal:
Curr Protoc Neurosci
Year:
2015
Document type:
Article
Affiliation country:
Switzerland
Publication country:
EEUU
/
ESTADOS UNIDOS
/
ESTADOS UNIDOS DA AMERICA
/
EUA
/
UNITED STATES
/
UNITED STATES OF AMERICA
/
US
/
USA