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Suppression of ornithine decarboxylase promotes osteogenic differentiation of human bone marrow-derived mesenchymal stem cells.
Tsai, Yo-Hsian; Lin, Kuan-Lian; Huang, Yuan-Pin; Hsu, Yi-Chiang; Chen, Chung-Hwan; Chen, Yuhsin; Sie, Min-Hua; Wang, Gwo-Jaw; Lee, Mon-Juan.
Affiliation
  • Tsai YH; Department of Bioscience Technology, Chang Jung Christian University, Tainan, Taiwan.
  • Lin KL; Department of Bioscience Technology, Chang Jung Christian University, Tainan, Taiwan.
  • Huang YP; Department of Cosmetics and Fashion Styling, Cheng Shiu University, Kaohsiung, Taiwan.
  • Hsu YC; Taiwan Graduate Institute of Medical Sciences, Chang Jung Christian University, Tainan, Taiwan.
  • Chen CH; Orthopaedic Research Center, Kaohsiung Medical University, Kaohsiung, Taiwan; Department of Orthopaedics, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan.
  • Chen Y; Taichung District Agricultural Research and Extension Station, Council of Agriculture, Executive Yuan, Taichung, Taiwan.
  • Sie MH; Department of Bioscience Technology, Chang Jung Christian University, Tainan, Taiwan.
  • Wang GJ; Orthopaedic Research Center, Kaohsiung Medical University, Kaohsiung, Taiwan; Department of Orthopaedics, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan; Department of Orthopaedics, National Cheng Kung University Hospital, Tainan, Taiwan; Department of Orthope
  • Lee MJ; Department of Bioscience Technology, Chang Jung Christian University, Tainan, Taiwan; Innovative Research Center of Medicine, Chang Jung Christian University, Tainan, Taiwan. Electronic address: mjlee@mail.cjcu.edu.tw.
FEBS Lett ; 589(16): 2058-65, 2015 Jul 22.
Article in En | MEDLINE | ID: mdl-26140984
ABSTRACT
Ornithine decarboxylase (ODC) is the rate-limiting enzyme for polyamine biosynthesis. Suppression of ODC by its irreversible inhibitor, α-difluoromethylornithine (DFMO), or by RNA interference through siRNA, enhanced osteogenic gene expression and alkaline phosphatase activity, and accelerated matrix mineralization of human bone marrow-derived mesenchymal stem cells (hBMSCs). Besides, adipogenic gene expression and lipid accumulation was attenuated, indicating that the enhanced osteogenesis was accompanied by down-regulation of adipogenesis when ODC was suppressed. A decrease in the intracellular polyamine content of hBMSCs during osteogenic induction was observed, suggesting that the level of endogenous polyamines is regulated during differentiation of hBMSCs. This study elucidates the role of polyamine metabolism in the lineage commitment of stem cells and provides a potential new indication for DFMO as bone-stimulating drug.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Ornithine Decarboxylase / Osteogenesis / Mesenchymal Stem Cells Limits: Humans Language: En Journal: FEBS Lett Year: 2015 Document type: Article Affiliation country: Taiwan
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Ornithine Decarboxylase / Osteogenesis / Mesenchymal Stem Cells Limits: Humans Language: En Journal: FEBS Lett Year: 2015 Document type: Article Affiliation country: Taiwan