Recent advances in genome editing and creation of genetically modified pigs.

Butler, James R; Ladowski, Joseph M; Martens, Gregory R; Tector, Matthew; Tector, A Joseph

Butler, James R; Ladowski, Joseph M; Martens, Gregory R; Tector, Matthew; Tector, A Joseph.

Affiliation

Butler JR; Department of Surgery, Indiana University School of Medicine, Indianapolis, IN, USA.
Ladowski JM; Department of Surgery, Indiana University School of Medicine, Indianapolis, IN, USA.
Martens GR; Department of Surgery, Indiana University School of Medicine, Indianapolis, IN, USA.
Tector M; Department of Surgery, Indiana University School of Medicine, Indianapolis, IN, USA.
Tector AJ; Department of Surgery, Indiana University School of Medicine, Indianapolis, IN, USA. Electronic address: jtector@iupui.edu.

Int J Surg ; 23(Pt B): 217-222, 2015 Nov.

Article in En | MEDLINE | ID: mdl-26231992

ABSTRACT

The field of xenotransplantation is benefiting greatly from recent advances in genetic engineering. The efficiency and pace with which new model animals are being created has dramatically sped progress towards clinical relevance. Endonuclease-driven genome editing now allows for the efficient generation of targeted genetic alterations. Herein we review the available methods of genetic engineering that have been successfully employed to create genetically modified pigs.

Subject(s)

Animals, Genetically Modified/genetics; Genetic Engineering/methods; Genome; Swine/genetics; Animals; Transplantation, Heterologous

Key words

Endonuclease; Homologous recombination; Off-target; TALEN CRISPR/Cas9; Zinc finger nuclease

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Swine / Animals, Genetically Modified / Genetic Engineering / Genome Limits: Animals Language: En Journal: Int J Surg Year: 2015 Document type: Article Affiliation country: United States Country of publication: United States

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