Global Analysis of Protein Expression and Phosphorylation Levels in Nicotine-Treated Pancreatic Stellate Cells.

Paulo, Joao A; Gaun, Aleksandr; Gygi, Steven P

Paulo, Joao A; Gaun, Aleksandr; Gygi, Steven P.

Affiliation

Paulo JA; Department of Cell Biology, Harvard Medical School , Boston, Massachusetts 02115, United States.
Gaun A; Department of Cell Biology, Harvard Medical School , Boston, Massachusetts 02115, United States.
Gygi SP; Department of Cell Biology, Harvard Medical School , Boston, Massachusetts 02115, United States.

J Proteome Res ; 14(10): 4246-56, 2015 Oct 02.

Article in En | MEDLINE | ID: mdl-26265067

Subject(s)

Nicotine/pharmacology; Pancreatic Stellate Cells/chemistry; Phosphoproteins/genetics; Protein Processing, Post-Translational; Proteome/genetics; Receptors, Nicotinic/genetics; Bungarotoxins/pharmacology; Cell Line; Cell Line, Tumor; Chromatography, Liquid; Gene Expression; Humans; Ligands; Neurons/chemistry; Neurons/cytology; Neurons/drug effects; Neurons/metabolism; Pancreatic Stellate Cells/cytology; Pancreatic Stellate Cells/drug effects; Pancreatic Stellate Cells/metabolism; Phosphoproteins/isolation & purification; Phosphoproteins/metabolism; Phosphorylation; Proteome/metabolism; Receptors, Nicotinic/metabolism; Tandem Mass Spectrometry

Key words

Fusion; SPS; Tandem mass tags; multiplexing; pancreatic cancer; pancreatitis; phosphopeptide enrichment; synchronous precursor selection

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Phosphoproteins / Protein Processing, Post-Translational / Receptors, Nicotinic / Proteome / Pancreatic Stellate Cells / Nicotine Type of study: Risk_factors_studies Limits: Humans Language: En Journal: J Proteome Res Journal subject: BIOQUIMICA Year: 2015 Document type: Article Affiliation country: United States Country of publication: United States

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