Oat ß-glucan ameliorates dextran sulfate sodium (DSS)-induced ulcerative colitis in mice.

ABSTRACT

Ulcerative colitis is a major inflammatory bowel disease (IBD), characterized by inflammation within the gastrointestinal tract through chronic or relapsing immune system activation. The aim of this study is to investigate the potential protective effect of oat ß-glucan (ßG) against colitis induced by DSS in mice. Eighty mice were randomly divided into the control group (no DSS, no ßG), DSS group (DSS only), DSS + L-ßG group (DSS plus 500 mg per kg ßG), and DSS + H-ßG group (DSS plus 1000 mg per kg ßG). Compared with the DSS group, administration of ßG significantly reduced clinical symptoms with less weight loss, diarrhea and shortening of the colon, the severity of colitis was significantly inhibited as evidenced by the reduced disease activity index (DAI) and degree of histological damage in colon. Moreover, treatment with ßG not only decreased myeloperoxidase activity (MPO), and nitric oxide (NO) and malondialdehyde (MDA) levels, but also inhibited mRNA and protein expression of pro-inflammatory factors such as TNF-α, IL-1ß, IL-6 and iNOS. This suggests that oat ßG in diet might exhibit an anti-inflammatory function against colitis through inhibition of expression of pro-inflammatory factors.