Optimization of Droplet Digital PCR from RNA and DNA extracts with direct comparison to RT-qPCR: Clinical implications for quantification of Oseltamivir-resistant subpopulations.

Taylor, Sean C; Carbonneau, Julie; Shelton, Dawne N; Boivin, Guy

Taylor, Sean C; Carbonneau, Julie; Shelton, Dawne N; Boivin, Guy.

Affiliation

Taylor SC; Bio-Rad Laboratories Canada, Inc., 1329 Meyerside Drive, Mississauga, ON, Canada L5T1C9. Electronic address: sean_taylor@bio-rad.com.
Carbonneau J; CHU of Quebec and Laval University, Quebec City, QC, Canada.
Shelton DN; Digital Biology Center, Bio-Rad Laboratories, Pleasanton, CA, USA.
Boivin G; CHU of Quebec and Laval University, Quebec City, QC, Canada.

J Virol Methods ; 224: 58-66, 2015 Nov.

Article in En | MEDLINE | ID: mdl-26315318

Subject(s)

Antiviral Agents/pharmacology; Drug Resistance, Viral; Influenza A Virus, H1N1 Subtype/drug effects; Influenza A Virus, H1N1 Subtype/genetics; Oseltamivir/pharmacology; Polymerase Chain Reaction/methods; Viral Load/methods; Child, Preschool; Humans; Influenza A Virus, H1N1 Subtype/classification; Influenza A Virus, H1N1 Subtype/isolation & purification; Influenza, Human/virology; Male; Neuraminidase/genetics; Reproducibility of Results; Sensitivity and Specificity; Viral Proteins/genetics

Key words

Droplet Digital PCR; Experimental optimization; Influenza virus; RT-qPCR; ddPCR

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Antiviral Agents / Polymerase Chain Reaction / Viral Load / Drug Resistance, Viral / Influenza A Virus, H1N1 Subtype / Oseltamivir Type of study: Diagnostic_studies / Evaluation_studies / Prognostic_studies Limits: Child, preschool / Humans / Male Language: En Journal: J Virol Methods Year: 2015 Document type: Article Country of publication: Netherlands

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