Effects of HMGB1 on proliferation and apoptosis of human brain glioma CD133 cells.

ABSTRACT

OBJECTIVE: To understand the effects of HMGB1 recombinant lentivirus vector on proliferation and apoptosis on CD133 cells after transfecting a human glioma cell strain CD133. METHOD: The CD133 human glioma cell strain cultured in vitro was transfected with HMGB1 lentiviral vector and empty vector, respectively, while CD133 cells cultured normally were used as the blank control. Changes in HMGB1 protein level were detected with western blot hybridization; apoptosis was detected with a flow cytometer; the cell proliferation was analysed with MTT method. RESULTS: The results from the western blot hybridization indicated that the expression level of HMGB1 protein in the group infected with HMGB1 recombinant over-expressed lentiviral vector increased significantly (p<0.05) compared with the blank control and CD133 cells transfected with the empty vector. The absorbance value of cell proliferation in the group infected with HMGB1 over-expressed lentiviral vector increased gradually on days 1, 2, 3, 4 and 5, but the difference was significant on day 2 after infection and later compared with the blank control group and negative control group (p<0.05). Based on detection with a flow cytometer, the apoptosis rate of CD133 cells transfected with HMGB1 over-expressed viral vector was significantly higher than that in the blank control group and negative control group (p<0.05). CONCLUSION: The lentiviral expression vector has very high transfection efficiency in human glioma cell line CD133 and the infection is able to significantly up-regulate the expression and activation of HMGB1. Over-expression of HMGB1 is able to inhibit proliferation of tumor cells and promote apoptosis (Tab. 2, Fig. 6, Ref. 30).