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Time interval after ischaemic preconditioning affects neuroprotection and gliosis in the gerbil hippocampal CA1 region induced by transient cerebral ischaemia.
Hye Kim, In; Lee, Jae-Chul; Ha Park, Joon; Hyeon Ahn, Ji; Cho, Jeong-Hwi; Hui Chen, Bai; Na Shin, Bich; Chun Yan, Bing; Rueol Ryu, Dong; Hong, Seongkweon; Hwi Cho, Jun; Lyul Lee, Yun; Kim, Young-Myeong; Cho, Byung-Ryul; Won, Moo-Ho.
Affiliation
  • Hye Kim I; a Department of Neurobiology, School of Medicine , Kangwon National University , Chuncheon , South Korea.
  • Lee JC; a Department of Neurobiology, School of Medicine , Kangwon National University , Chuncheon , South Korea.
  • Ha Park J; a Department of Neurobiology, School of Medicine , Kangwon National University , Chuncheon , South Korea.
  • Hyeon Ahn J; a Department of Neurobiology, School of Medicine , Kangwon National University , Chuncheon , South Korea.
  • Cho JH; a Department of Neurobiology, School of Medicine , Kangwon National University , Chuncheon , South Korea.
  • Hui Chen B; b Department of Physiology, College of Medicine , Hallym University , Chuncheon , South Korea.
  • Na Shin B; b Department of Physiology, College of Medicine , Hallym University , Chuncheon , South Korea.
  • Chun Yan B; c Institute of Integrative Traditional & Western Medicine, Medical College , Yangzhou University , China.
  • Rueol Ryu D; d Department of Internal Medicine, School of Medicine , Kangwon National University , Chuncheon , South Korea.
  • Hong S; e Department of Surgery, School of Medicine , Kangwon National University , Chuncheon , South Korea.
  • Hwi Cho J; f Department of Emergency Medicine, School of Medicine , Kangwon National University , Chuncheon , South Korea.
  • Lyul Lee Y; b Department of Physiology, College of Medicine , Hallym University , Chuncheon , South Korea.
  • Kim YM; g Department of Molecular and Cellular Biochemistry, School of Medicine , Kangwon National University , Chuncheon , South Korea.
  • Cho BR; d Department of Internal Medicine, School of Medicine , Kangwon National University , Chuncheon , South Korea.
  • Won MH; a Department of Neurobiology, School of Medicine , Kangwon National University , Chuncheon , South Korea.
Neurol Res ; 38(3): 210-9, 2016 Mar.
Article in En | MEDLINE | ID: mdl-26352929
ABSTRACT

OBJECTIVES:

Ischaemic preconditioning (IPC) can increase ischaemic tolerance of the central nervous system (CNS) to a subsequent longer or lethal period of transient ischaemia. In this study, we examined neuroprotective effects of time intervals after IPC against ischaemic insult in the hippocampus.

METHODS:

Animals were randomly assigned to six groups; sham-operated-group, ischaemia-operated-group, and three IPC (12 hours, 1- and 2-day intervals after IPC) plus ischaemia-groups (IPC-12 hour, 1 and 2-day interval-ischaemia-operated-groups). For neuroprotection, we carried out cresyl violet (CV) staining neuronal nuclei (NeuN) immunohistochemistry and Fluoro-Jade B histofluorescence staining. In addition, we examined gliosis using immunohistochemistry for GFAP (a marker for astrocytes) and Iba-1 (a marker for microglia).

RESULTS:

A significant loss of neurons was observed in the stratum pyramidale (SP) of the hippocampal CA1 region (CA1) in the ischaemia-operated-group and IPC-12 hours interval-ischaemia-operated-groups. In the IPC-1 day interval-ischaemia-operated-group, CA1 pyramidal neurons were well protected from ischaemic insult; the neuroprotective effect in the IPC-2 day interval-ischaemia-operated-group was less than that in the IPC-1 day interval-ischaemia-operated-group. On the other hand, we observed changes in glial cells (astrocytes and microglia) in the CA1 of all groups. The distribution pattern of glial cells only in the IPC-1 day interval-ischaemia-operated-group was similar to that in the sham-group.

CONCLUSION:

In brief, our findings indicate that 1 day after IPC displays a mighty neuroprotection and shows an inhibition of glial activation in the CA1 induced by transient ischaemic insult.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Ischemic Attack, Transient / Ischemic Preconditioning / CA1 Region, Hippocampal / Gliosis Type of study: Prognostic_studies Limits: Animals Language: En Journal: Neurol Res Year: 2016 Document type: Article Affiliation country: South Korea
Fulltext
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Ischemic Attack, Transient / Ischemic Preconditioning / CA1 Region, Hippocampal / Gliosis Type of study: Prognostic_studies Limits: Animals Language: En Journal: Neurol Res Year: 2016 Document type: Article Affiliation country: South Korea