A protein kinase binds the C-terminal domain of the readthrough protein of Turnip yellows virus and regulates virus accumulation.
Virology
; 486: 44-53, 2015 Dec.
Article
in En
| MEDLINE
| ID: mdl-26402374
ABSTRACT
Turnip yellows virus (TuYV), a phloem-limited virus, encodes a 74kDa protein known as the readthrough protein (RT) involved in virus movement. We show here that a TuYV mutant deleted of the C-terminal part of the RT protein (TuYV-∆RTCter) was affected in long-distance trafficking in a host-specific manner. By using the C-terminal domain of the RT protein as a bait in a yeast two-hybrid screen of a phloem cDNA library from Arabidopsis thaliana we identified the calcineurin B-like protein-interacting protein kinase-7 (AtCIPK7). Transient expression of a GFPCIPK7 fusion protein in virus-inoculated Nicotiana benthamiana leaves led to local increase of wild-type TuYV accumulation, but not that of TuYV-∆RTCter. Surprisingly, elevated virus titer in inoculated leaves did not result in higher TuYV accumulation in systemic leaves, which indicates that virus long-distance movement was not affected. Since GFPCIPK7 was localized in or near plasmodesmata, CIPK7 could negatively regulate TuYV export from infected cells.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Plant Diseases
/
Protein Kinases
/
Viral Proteins
/
Protein Serine-Threonine Kinases
/
Arabidopsis
/
Arabidopsis Proteins
/
Luteoviridae
Language:
En
Journal:
Virology
Year:
2015
Document type:
Article
Affiliation country:
France