Urate crystals induce NLRP3 inflammasome-dependent IL-1ß secretion and proliferation in isolated primary human T-cells.
Hippokratia
; 19(1): 41-6, 2015.
Article
in En
| MEDLINE
| ID: mdl-26435646
ABSTRACT
BACKGROUND:
Urate through NOD-like receptor family, pyrin domain containing 3 (NLRP3) inflammasome-dependent caspase-1 activation stimulates macrophages to secrete inteleukin-1ß (IL-1ß). Urate also enhances adaptive immunity indirectly through its effect on antigen presenting cells. In this study, the direct effect of urate on isolated primary human T-cells was evaluated.METHODS:
Isolated T-cells were cultured with or without monosodium urate crystals in the presence or not of the NLRP3 inflammasome inhibitor glyburide. Activated cleaved caspase-1 was assessed by means of western blotting, whereas caspase-1 activity was measured colorimetrically in the cell lysates. IL-1ß was measured in the supernatants by means of enzyme-linked immunosorbent assay. T-cell proliferation was assessed by means of bromodeoxyuridine labelling and immunoenzymatic detection.RESULTS:
Urate induced caspase-1 activation and IL-1ß release by T-cells. It also induced proliferation of T-cells. Glyburide inhibited urate-induced caspase-1 activation, IL-1ß secretion and proliferation.CONCLUSIONS:
Urate, a well defined danger signal, stimulates directly human T-cells in a NLRP3 infmmasomela-dependent way. The subsequent IL-1ß secretion could enhance inflammation, whereas expansion of T-cell clones could facilitate a subsequent adaptive immune response. Hippokratia 2015, 19 (1) 41-46.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Language:
En
Journal:
Hippokratia
Year:
2015
Document type:
Article
Affiliation country:
Greece