[Use of mass spectrometry for rapid diagnosis of positive blood cultures: Validation of a local algorythm]. / Utilización de espectrometría de masas para la identificación precoz de hemocultivos positivos: Validación de un algoritmo local.
Rev Chilena Infectol
; 32(4): 399-402, 2015 Aug.
Article
in Es
| MEDLINE
| ID: mdl-26436783
ABSTRACT
Mass spectrometry (MS) is used in identification of positive blood culture, a contribution in the clinical management of septic patients. The protocol is labor intensive and disrupts the normal workflow in a clinical laboratory. We intended to make rapid diagnosis by using MS directly from shortly incubated blood agar plates (4 to 6 hours) comparing with results of the conventional method (MC). We worked in parallel 145 positive blood cultures, with correct identification by short method in 79% of cases. We observed better yield with non carbon bottles and with gramnegative rods. With this information we designed a rapid identification algorithm using MS, which allows advancing diagnosis in 12-16 hours, without increasing to the costs or work load, since extraction protocol is not used.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Mass Spectrometry
/
Algorithms
/
Bacteriological Techniques
Type of study:
Diagnostic_studies
/
Guideline
/
Prognostic_studies
Language:
Es
Journal:
Rev Chilena Infectol
Year:
2015
Document type:
Article