Nanotherapeutics with anthracyclines: methods of determination and quantification of anthracyclines in biological samples.
Physiol Res
; 64(Suppl 1): S1-10, 2015.
Article
in En
| MEDLINE
| ID: mdl-26447590
ABSTRACT
Anthracyclines, e.g. doxorubicin, pirarubicin, are widely used as cytostatic agents in the polymer nanotherapeutics designed for the highly effective antitumor therapy with reduced side effects. However, their precise dosage scheme needs to be optimized, which requires an accurate method for their quantification on the cellular level in vitro during nanocarrier development and in body fluids and tissues during testing in vivo. Various methods detecting the anthracycline content in biological samples have already been designed. Most of them are highly demanding and they differ in exactness and reproducibility. The cellular uptake and localization is predominantly observed and determined by microscopy techniques, the anthracycline content is usually quantified by chromatographic analysis using fluorescence detection. We reviewed and compared published methods concerning the detection of anthracycline nanocarriers.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Anthracyclines
/
Nanomedicine
/
Antineoplastic Agents
Limits:
Humans
Language:
En
Journal:
Physiol Res
Journal subject:
FISIOLOGIA
Year:
2015
Document type:
Article
Affiliation country:
Czech Republic