n-TiO2 and CdCl2 co-exposure to titanium dioxide nanoparticles and cadmium: Genomic, DNA and chromosomal damage evaluation in the marine fish European sea bass (Dicentrarchus labrax).
Aquat Toxicol
; 168: 72-7, 2015 Nov.
Article
in En
| MEDLINE
| ID: mdl-26448269
ABSTRACT
Due to the large production and growing use of titanium dioxide nanoparticles (n-TiO2), their release in the marine environment and their potential interaction with existing toxic contaminants represent a growing concern for biota. Different end-points of genotoxicity were investigated in the European sea bass Dicentrarchus labrax exposed to n-TiO2 (1mgL(-1)) either alone and combined with CdCl2 (0.1mgL(-1)) for 7 days. DNA primary damage (comet assay), apoptotic cells (diffusion assay), occurrence of micronuclei and nuclear abnormalities (cytome assay) were assessed in peripheral erythrocytes and genomic stability (random amplified polymorphism DNA-PCR, RAPD assay) in muscle tissue. Results showed that genome template stability was reduced after CdCl2 and n-TiO2 exposure. Exposure to n-TiO2 alone was responsible for chromosomal alteration but ineffective in terms of DNA damage; while the opposite was observed in CdCl2 exposed specimens. Co-exposure apparently prevents the chromosomal damage and leads to a partial recovery of the genome template stability.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Water Pollutants, Chemical
/
Bass
/
DNA Damage
/
DNA
/
Chromosomes
/
Genome
/
Nanoparticles
Limits:
Animals
Language:
En
Journal:
Aquat Toxicol
Journal subject:
BIOLOGIA
/
TOXICOLOGIA
Year:
2015
Document type:
Article
Affiliation country:
Italy