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Disulfide-Bridged Cleavable PEGylation of Poly-L-Lysine for SiRNA Delivery.
Tang, Min; Dong, Haiqing; Cai, Xiaojun; Zhu, Haiyan; Ren, Tianbin; Li, Yongyong.
Affiliation
  • Tang M; Shanghai East Hospital, The Institute for Biomedical Engineering & Nano Science (iNANO), Tongji University School of Medicine, Shanghai, China.
  • Dong H; Shanghai East Hospital, The Institute for Biomedical Engineering & Nano Science (iNANO), Tongji University School of Medicine, Shanghai, China.
  • Cai X; Shanghai East Hospital, The Institute for Biomedical Engineering & Nano Science (iNANO), Tongji University School of Medicine, Shanghai, China.
  • Zhu H; Shanghai East Hospital, The Institute for Biomedical Engineering & Nano Science (iNANO), Tongji University School of Medicine, Shanghai, China.
  • Ren T; Shanghai East Hospital, The Institute for Biomedical Engineering & Nano Science (iNANO), Tongji University School of Medicine, Shanghai, China.
  • Li Y; Shanghai East Hospital, The Institute for Biomedical Engineering & Nano Science (iNANO), Tongji University School of Medicine, Shanghai, China. yongyong_li@tongji.edu.cn.
Methods Mol Biol ; 1364: 49-61, 2016.
Article in En | MEDLINE | ID: mdl-26472441
Engineered PEG-cleavable catiomers based on poly-L-lysine have been developed as nonviral gene vectors, which have been found to be one of important methods to balance "PEG dilemma." In this protocol, we aim at the standardization of the method and procedure of PEG-cleavable catiomers. Major steps including ring-opening polymerization (ROP) of ε-benzyloxycarbonyl-L-lysine N-carboxyanhydride (zLL-NCA) monomers to yield PEG-cleavable polylysine, examination on bio-stability and bio-efficacy of its gene complexes are described.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Polyethylene Glycols / Polylysine / Drug Carriers / RNA, Small Interfering / Disulfides Limits: Humans Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2016 Document type: Article Affiliation country: China Country of publication: United States
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Polyethylene Glycols / Polylysine / Drug Carriers / RNA, Small Interfering / Disulfides Limits: Humans Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2016 Document type: Article Affiliation country: China Country of publication: United States