From Enzyme to Whole Blood: Sequential Screening Procedure for Identification and Evaluation of p38 MAPK Inhibitors.

Bauer, Silke M; Kubiak, Jakub M; Rothbauer, Ulrich; Laufer, Stefan

Bauer, Silke M; Kubiak, Jakub M; Rothbauer, Ulrich; Laufer, Stefan.

Affiliation

Bauer SM; Department of Pharmaceutical and Medicinal Chemistry, Institute of Pharmacy, Eberhard-Karls-University Teubingen, Auf der Morgenstelle 8, 72076, Tuebingen, Germany.
Kubiak JM; Department of Pharmaceutical and Medicinal Chemistry, Institute of Pharmacy, Eberhard-Karls-University Teubingen, Auf der Morgenstelle 8, 72076, Tuebingen, Germany.
Rothbauer U; Department of Pharmaceutical and Medicinal Chemistry, Institute of Pharmacy, Eberhard-Karls-University Teubingen, Auf der Morgenstelle 8, 72076, Tuebingen, Germany.
Laufer S; Department of Pharmaceutical and Medicinal Chemistry, Institute of Pharmacy, Eberhard-Karls-University Teubingen, Auf der Morgenstelle 8, 72076, Tuebingen, Germany. stefan.laufer@uni-tuebingen.de.

Methods Mol Biol ; 1360: 123-48, 2016.

Article in En | MEDLINE | ID: mdl-26501907

Subject(s)

High-Throughput Screening Assays/methods; Protein Kinase Inhibitors/isolation & purification; p38 Mitogen-Activated Protein Kinases/antagonists & inhibitors; Blood Proteins/metabolism; Drug Discovery/methods; Enzyme-Linked Immunosorbent Assay/methods; Genes, Reporter; Green Fluorescent Proteins/analysis; Green Fluorescent Proteins/genetics; Humans; Indicators and Reagents; Intracellular Signaling Peptides and Proteins/genetics; Intracellular Signaling Peptides and Proteins/metabolism; Lipopolysaccharides/pharmacology; Protein Binding; Protein Kinase Inhibitors/pharmacology; Protein Serine-Threonine Kinases/genetics; Protein Serine-Threonine Kinases/metabolism; Protein Transport; Recombinant Fusion Proteins/analysis; Recombinant Fusion Proteins/metabolism; Structure-Activity Relationship; Substrate Specificity; p38 Mitogen-Activated Protein Kinases/blood; p38 Mitogen-Activated Protein Kinases/metabolism

Key words

Cell-based assay; ELISA; Human whole-blood; Inhibitor screening; MK2-EGFP translocation assay; p38 MAPK

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: P38 Mitogen-Activated Protein Kinases / Protein Kinase Inhibitors / High-Throughput Screening Assays Type of study: Diagnostic_studies / Prognostic_studies / Screening_studies Limits: Humans Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2016 Document type: Article Affiliation country: Germany Country of publication: United States

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