Methodology for preservation of yeast-bound single chain fragment variable antibody affinity reagents.
J Immunol Methods
; 427: 134-7, 2015 Dec.
Article
in En
| MEDLINE
| ID: mdl-26571425
ABSTRACT
Readily accessible affinity reagents are critical to the validation of biomarkers and to the development of new diagnostic tests. As alternatives to monoclonal antibodies, yeast-bound single chain fragment variable antibody (yeast-scFv) can be rapidly selected from yeast display libraries. An important characteristic for any diagnostic reagent is its stability or ability to store it. A lyophilization procedure that has extended the shelf life of yeast-scFv by a factor of ≥10-fold relative to previous reports is reported. Real time stability for three yeast-scFv clones to three distinct Entamoeba histolytica potential diagnostic antigen targets for one year at room temperature as well as at 37°C and 45°C. Retention of full binding activity and specificity of the yeast-scFv clones for their cognate antigens is shown by flow cytometry. Lyophilization can easily be carried out in batches and in single-use vials.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Reagent Kits, Diagnostic
/
Drug Stability
/
Drug Storage
/
Single-Chain Antibodies
Language:
En
Journal:
J Immunol Methods
Year:
2015
Document type:
Article
Affiliation country:
United States