Conditioned medium from human bone marrow-derived mesenchymal stem cells promotes skin moisturization and effacement of wrinkles in UVB-irradiated SKH-1 hairless mice.
Photodermatol Photoimmunol Photomed
; 32(3): 120-8, 2016 May.
Article
in En
| MEDLINE
| ID: mdl-26577060
ABSTRACT
BACKGROUND:
Mesenchymal stem cells (MSCs) are promising therapeutic agents for various diseases.AIMS:
To investigate the effects of conditioned medium from human bone marrow-derived mesenchymal stem cells (MSC-CdM) on pro-collagen production and wrinkle formation, we performed in vitro and in vivo experiments.METHODS:
We assessed the effects of MSC-CdM on proliferation and photo-aging in human dermal fibroblasts after UVB exposure using enzyme activity assays for collagen type I secretion and MMP-1. To determine the effect of topically applied MSC-CdM on wrinkle formation, MSC-CdM (1% and 10%) and vehicle (propylene glycol ethanol, 7 3) were applied to the dorsal skin of UVB-irradiated hairless mice for 8 weeks. We examined the effects on wrinkle formation by assessing visual skin grading, replica, tape stripping, transepidermal water loss (TEWL), and skin hydration measurement. We also examined histology of the lesions using hematoxylin-eosin, Masson's trichrome, and immunohistochemical staining.RESULTS:
MSC-CdM markedly reduced UV-induced matrix metalloproteinase-1 expression and increased pro-collagen synthesis in a dose-dependent manner. Our findings suggest that MSC-CdM induces repair of dermal damage and effacement of wrinkles on UVB-irradiated hairless mice through protective effect of hydration.CONCLUSION:
These results support an anti-wrinkle effect of MSC-CdM that involves increased collagen synthesis and suggest that MSC-CdM might be a potential candidate for preventing UV-induced skin damage.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Skin
/
Skin Aging
/
Mesenchymal Stem Cells
Limits:
Animals
/
Female
/
Humans
/
Male
Language:
En
Journal:
Photodermatol Photoimmunol Photomed
Journal subject:
ALERGIA E IMUNOLOGIA
/
DERMATOLOGIA
Year:
2016
Document type:
Article