Evaluation of the Proliferative Effects Induced by Low-Level Laser Therapy in Bone Marrow Stem Cell Culture.
Photomed Laser Surg
; 33(12): 610-6, 2015 Dec.
Article
in En
| MEDLINE
| ID: mdl-26580583
ABSTRACT
OBJECTIVE:
The objective of this study was to evaluate the effect of laser irradiation on dog bone marrow stem cells. BACKGROUND DATA Low doses of low-level red laser positively affect the viability of mesenchymal stem cells, and also increase proliferation.METHODS:
Low-level laser (wavelength, 660 nm; power output, 50 mW), was applied to dog bone marrow stem cell cultures (DBMSC). The energy densities delivered varied from 1 to 12J/cm(2). The effect of the laser irradiation was evaluated on cell proliferation measured with the MTT colorimetric test, cell cycle phase, and on lipidic peroxidation (free radical production).RESULTS:
The results indicate that laser irradiation to DBMSC did not change the morphology of the cells, but significantly increased their viability and the number of cells at the G2/M phase with 6, 10, and 12 J/cm(2). On the other hand, malonaldehyde production was significantly enhanced with 8 J/cm(2).CONCLUSIONS:
The parameters used to irradiate DBMSC increased significantly proliferation without producing high levels of reactive oxygen species (ROS).
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Low-Level Light Therapy
/
Cell Proliferation
/
Mesenchymal Stem Cells
Limits:
Animals
/
Humans
Language:
En
Journal:
Photomed Laser Surg
Year:
2015
Document type:
Article
Affiliation country:
Brazil