Your browser doesn't support javascript.
loading
Comparative Characterization of Vibrio cholerae O1 from Five Sub-Saharan African Countries Using Various Phenotypic and Genotypic Techniques.
Smith, Anthony M; Njanpop-Lafourcade, Berthe-Marie; Mengel, Martin A; Gessner, Bradford D; Sauvageot, Delphine; Bidjada, Bawimodom; Miwanda, Berthe N; Saliou, Diallo M; N'Douba, Adèle Kacou; Langa, José P; Ismail, Husna; Tau, Nomsa; Sooka, Arvinda; Keddy, Karen H.
Affiliation
  • Smith AM; Centre for Enteric Diseases, National Institute for Communicable Diseases, Division in the National Health Laboratory Service (NHLS), Johannesburg, South Africa.
  • Njanpop-Lafourcade BM; Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa.
  • Mengel MA; Agence de Médecine Préventive (AMP), Paris, France.
  • Gessner BD; Agence de Médecine Préventive (AMP), Paris, France.
  • Sauvageot D; Agence de Médecine Préventive (AMP), Paris, France.
  • Bidjada B; Agence de Médecine Préventive (AMP), Paris, France.
  • Miwanda BN; Institut National d'Hygiène, Lomé, Togo.
  • Saliou DM; Institut National d'Hygiène, Lomé, Togo.
  • N'Douba AK; Institut National de Recherche Biomédicale, Kinshasa, Democratic Republic of Congo.
  • Langa JP; Institut National de Santé Publique, Conakry, Guinea.
  • Ismail H; Institut Pasteur de Côte d'Ivoire, Abidjan, Côte d'Ivoire.
  • Tau N; National Institute of Health, Maputo, Mozambique.
  • Sooka A; Centre for Enteric Diseases, National Institute for Communicable Diseases, Division in the National Health Laboratory Service (NHLS), Johannesburg, South Africa.
  • Keddy KH; Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa.
PLoS One ; 10(11): e0142989, 2015.
Article in En | MEDLINE | ID: mdl-26606536
ABSTRACT
We used standardized methodologies to characterize Vibrio cholerae O1 isolates from Guinea, Democratic Republic of the Congo (DRC), Togo, Côte d'Ivoire and Mozambique. We investigated 257 human isolates collected in 2010 to 2013. DRC isolates serotyped O1 Inaba, while isolates from other countries serotyped O1 Ogawa. All isolates were biotype El Tor and positive for cholera toxin. All isolates showed multidrug resistance but lacked ciprofloxacin resistance. Antimicrobial susceptibility profiles of isolates varied between countries. In particular, the susceptibility profile of isolates from Mozambique (East-Africa) included resistance to ceftriaxone and was distinctly different to the susceptibility profiles of isolates from countries located in West- and Central-Africa. Molecular subtyping of isolates using pulsed-field gel electrophoresis (PFGE) analysis showed a complex relationship among isolates. Some PFGE patterns were unique to particular countries and clustered by country; while other PFGE patterns were shared by isolates from multiple countries, indicating that the same genetic lineage is present in multiple countries. Our data add to a better understanding of cholera epidemiology in Africa.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Phenotype / Cholera / Vibrio cholerae O1 / Genotype Type of study: Screening_studies Limits: Adolescent / Adult / Female / Humans / Male / Middle aged Country/Region as subject: Africa Language: En Journal: PLoS One Journal subject: CIENCIA / MEDICINA Year: 2015 Document type: Article Affiliation country: South Africa

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Phenotype / Cholera / Vibrio cholerae O1 / Genotype Type of study: Screening_studies Limits: Adolescent / Adult / Female / Humans / Male / Middle aged Country/Region as subject: Africa Language: En Journal: PLoS One Journal subject: CIENCIA / MEDICINA Year: 2015 Document type: Article Affiliation country: South Africa