Human papillomavirus types 16 E1 mRNA is transcribed from P14 early promoter in cervical neoplasms.
Virology
; 488: 196-201, 2016 Jan 15.
Article
in En
| MEDLINE
| ID: mdl-26655237
ABSTRACT
High-risk human papillomavirus (HR-HPV) persistent infection is responsible for the development of the majority of cervical cancers. The therapy against HPV-associated cancer requires knowledge of the viral gene expression mechanisms. In this study, the polyadenylated polycistronic transcripts containing full-size E1ORF and produced from the early P14 promoter were detected for the first time in cervical tumors with episomal forms of the HPV16 genome. P14-initiated mRNAs were revealed also in precancerous lesions. The amount of P14-initiated transcripts was significantly less compared to transcripts initiated from the major P97 HPV16 promoter in cervical intraepithelial neoplasms and squamous cell carcinomas. The ratios of P97/P14-transcripts determined by qRT-PCR were unique for each clinical sample and varied in quite wide ranges independent of disease progression stages or tumor grade. These data suggest that the levels of P14- and P97-transcripts are regulated independently from each other in cervical neoplasms.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Transcription, Genetic
/
RNA, Messenger
/
RNA, Viral
/
Uterine Cervical Neoplasms
/
Oncogene Proteins, Viral
/
Promoter Regions, Genetic
/
Human papillomavirus 16
Limits:
Female
/
Humans
Language:
En
Journal:
Virology
Year:
2016
Document type:
Article