Rapid NMR Assignments of Proteins by Using Optimized Combinatorial Selective Unlabeling.

Dubey, Abhinav; Kadumuri, Rajashekar Varma; Jaipuria, Garima; Vadrevu, Ramakrishna; Atreya, Hanudatta S

Dubey, Abhinav; Kadumuri, Rajashekar Varma; Jaipuria, Garima; Vadrevu, Ramakrishna; Atreya, Hanudatta S.

Affiliation

Dubey A; NMR Research Center, Indian Institute of Science, Malleswaram, Bangalore, 560012, India.
Kadumuri RV; IISc Mathematics Initiative, Indian Institute of Science, Malleswaram, Bangalore, 560012, India.
Jaipuria G; Department of Biological Sciences, Birla Institute of Technology and Science-Pilani, Hyderabad Campus, Hyderabad, 500078, India.
Vadrevu R; NMR Research Center, Indian Institute of Science, Malleswaram, Bangalore, 560012, India.
Atreya HS; Department of Biological Sciences, Birla Institute of Technology and Science-Pilani, Hyderabad Campus, Hyderabad, 500078, India. vrk@hyderabad.bits-pilani.ac.in.

Chembiochem ; 17(4): 334-40, 2016 Feb 15.

Article in En | MEDLINE | ID: mdl-26662553

Subject(s)

Nuclear Magnetic Resonance, Biomolecular/methods; Proteins/chemistry; Amino Acid Sequence; Amino Acids/analysis; Deuterium/analysis; Escherichia coli/enzymology; Humans; Insulin-Like Growth Factor Binding Protein 2/chemistry; Molecular Sequence Data; Nitrogen Isotopes/analysis; Tryptophan Synthase/chemistry

Key words

NMR spectroscopy; isotope scrambling; proteins; selective unlabeling assignments; sequence specific resonance; triple resonance experiments

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Proteins / Nuclear Magnetic Resonance, Biomolecular Type of study: Prognostic_studies Limits: Humans Language: En Journal: Chembiochem Journal subject: BIOQUIMICA Year: 2016 Document type: Article Affiliation country: India Country of publication: Germany

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