Laboratory Validation of the Sand Fly Fever Virus Antigen Assay.
J Am Mosq Control Assoc
; 31(4): 380-3, 2015 Dec.
Article
in En
| MEDLINE
| ID: mdl-26675463
ABSTRACT
Sandfly fever group viruses in the genus Phlebovirus (family Bunyaviridae) are widely distributed across the globe and are a cause of disease in military troops and indigenous peoples. We assessed the laboratory sensitivity and specificity of the Sand Fly Fever Virus Antigen Assay, a rapid dipstick assay designed to detect sandfly fever Naples virus (SFNV) and Toscana virus (TOSV) against a panel of phleboviruses. The assay detected SFNV and TOSV, as well as other phleboviruses including Aguacate, Anahanga, Arumowot, Chagres, and Punta Toro viruses. It did not detect sandfly fever Sicilian, Heartland, Rio Grande, or Rift Valley fever viruses. It did not produce false positive results in the presence of uninfected sand flies (Lutzomyia longipalpis) or Cache Valley virus, a distantly related bunyavirus. Results from this laboratory evaluation suggest that this assay may be used as a rapid field-deployable assay to detect sand flies infected with TOSV and SFNV, as well as an assortment of other phleboviruses.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Psychodidae
/
Immunoassay
/
Sandfly fever Naples virus
Limits:
Animals
Language:
En
Journal:
J Am Mosq Control Assoc
Journal subject:
MEDICINA TROPICAL
Year:
2015
Document type:
Article