Crosslinked hydrogels based on biological macromolecules with potential use in skin tissue engineering.
Int J Biol Macromol
; 84: 174-81, 2016 Mar.
Article
in En
| MEDLINE
| ID: mdl-26704998
ABSTRACT
Zero-length crosslinked hydrogels have been synthesized by covalent linking of three natural polymers (collagen, hyaluronic acid and sericin), in the presence of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide. The hydrogels have been investigated by FT-IR spectroscopy, microcalorimetry, in vitro swelling, enzymatic degradation, and in vitro cell viability studies. The obtained crosslinked hydrogels showed a macroporous structure, high swelling degree and in vitro enzymatic resistance compared to uncrosslinked collagen. The in vitro cell viability studies performed on normal human dermal fibroblasts assessed the sericin proliferation properties indicating a potential use of the hydrogels based on collagen, hyaluronic acid and sericin in skin tissue engineering.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Skin
/
Biocompatible Materials
/
Hydrogels
/
Tissue Engineering
Language:
En
Journal:
Int J Biol Macromol
Year:
2016
Document type:
Article