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Challenging the Roles of NSP3 and Untranslated Regions in Rotavirus mRNA Translation.
Gratia, Matthieu; Vende, Patrice; Charpilienne, Annie; Baron, Hilma Carolina; Laroche, Cécile; Sarot, Emeline; Pyronnet, Stéphane; Duarte, Mariela; Poncet, Didier.
Affiliation
  • Gratia M; Institut de Biologie Integrative de la Cellule (I2BC), UMR 9198, Département de Virologie, USC INRA 1358, Gif sur Yvette, France.
  • Vende P; Institut de Biologie Integrative de la Cellule (I2BC), UMR 9198, Département de Virologie, USC INRA 1358, Gif sur Yvette, France.
  • Charpilienne A; Institut de Biologie Integrative de la Cellule (I2BC), UMR 9198, Département de Virologie, USC INRA 1358, Gif sur Yvette, France.
  • Baron HC; Institut de Biologie Integrative de la Cellule (I2BC), UMR 9198, Département de Virologie, USC INRA 1358, Gif sur Yvette, France.
  • Laroche C; Institut de Biologie Integrative de la Cellule (I2BC), UMR 9198, Département de Virologie, USC INRA 1358, Gif sur Yvette, France.
  • Sarot E; INSERM UMR-1037 - Université de Toulouse III-Paul Sabatier, Laboratoire d'Excellence Toulouse Cancer (TOUCAN), Equipe labellisée Ligue Contre le Cancer Toulouse, France.
  • Pyronnet S; INSERM UMR-1037 - Université de Toulouse III-Paul Sabatier, Laboratoire d'Excellence Toulouse Cancer (TOUCAN), Equipe labellisée Ligue Contre le Cancer Toulouse, France.
  • Duarte M; Institut de Biologie Integrative de la Cellule (I2BC), UMR 9198, Département de Virologie, USC INRA 1358, Gif sur Yvette, France.
  • Poncet D; Université d'Evry Val d'Essonne, Département de Biologie, Evry, France.
PLoS One ; 11(1): e0145998, 2016.
Article in En | MEDLINE | ID: mdl-26727111
Rotavirus NSP3 is a translational surrogate of the PABP-poly(A) complex for rotavirus mRNAs. To further explore the effects of NSP3 and untranslated regions (UTRs) on rotavirus mRNAs translation, we used a quantitative in vivo assay with simultaneous cytoplasmic NSP3 expression (wild-type or deletion mutant) and electroporated rotavirus-like and standard synthetic mRNAs. This assay shows that the last four GACC nucleotides of viral mRNA are essential for efficient translation and that both the NSP3 eIF4G- and RNA-binding domains are required. We also show efficient translation of rotavirus-like mRNAs even with a 5'UTR as short as 5 nucleotides, while more than eleven nucleotides are required for the 3'UTR. Despite the weak requirement for a long 5'UTR, a good AUG environment remains a requirement for rotavirus mRNAs translation.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Protein Biosynthesis / RNA, Messenger / Viral Nonstructural Proteins / 3' Untranslated Regions / 5' Untranslated Regions Limits: Animals Language: En Journal: PLoS One Journal subject: CIENCIA / MEDICINA Year: 2016 Document type: Article Affiliation country: France Country of publication: United States

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Protein Biosynthesis / RNA, Messenger / Viral Nonstructural Proteins / 3' Untranslated Regions / 5' Untranslated Regions Limits: Animals Language: En Journal: PLoS One Journal subject: CIENCIA / MEDICINA Year: 2016 Document type: Article Affiliation country: France Country of publication: United States