Photodynamic detection of canine mammary gland tumours after oral administration of 5-aminolevulinic acid.
Vet Comp Oncol
; 15(3): 731-739, 2017 Sep.
Article
in En
| MEDLINE
| ID: mdl-26766288
ABSTRACT
5-Aminolevulinic acid (5-ALA) is widely used in photodynamic detection (PDD) and therapy. We evaluated the pharmacokinetics of 5-ALA-induced porphyrins and its effectiveness in PDD in dogs with mammary gland tumours (MGTs) following oral administration. Healthy dogs and those with MGTs (nine each) were orally administered 4 mg kg-1 5-ALA. Protoporphyrin IX (PpIX) was not detected in the plasma of healthy dogs but it peaked in dogs with MGT at 2 h after 5-ALA administration. In the PDD study, 16 dogs with MGT were orally administered 40 mg kg-1 5-ALA, and MGT but not normal tissue showed red fluorescence after 2-4 h. Photon counts were 6635-63 890 and 59-4011 (median, 19 943 and 919) for MGT and non-tumour tissues, respectively. Cell density strongly correlated with PpIX photon counts of MGT tissue of the dogs (R = 0.743, P = 0.0009). We suggest that 5-ALA-PDD might be an effective diagnostic tool for MGTs.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Spectrometry, Fluorescence
/
Mammary Neoplasms, Animal
/
Photosensitizing Agents
/
Dog Diseases
/
Aminolevulinic Acid
Type of study:
Diagnostic_studies
Limits:
Animals
Language:
En
Journal:
Vet Comp Oncol
Journal subject:
MEDICINA VETERINARIA
/
NEOPLASIAS
Year:
2017
Document type:
Article
Affiliation country:
Japan