Azospirillum agricola sp. nov., a nitrogen-fixing species isolated from cultivated soil.
Int J Syst Evol Microbiol
; 66(3): 1453-1458, 2016 Mar.
Article
in En
| MEDLINE
| ID: mdl-26786719
A polyphasic approach was used to characterize a novel nitrogen-fixing bacterial strain, designated CC-HIH038T, isolated from cultivated soil in Taiwan. Cells of strain CC-HIH038T were Gram-stain-negative, facultatively aerobic and spiral-shaped, with motility provided by a single polar flagellum. The 16S rRNA gene sequence analysis of strain CC-HIH038T showed highest sequence similarity to Azospirillum doebereinerae (98.0â%), Azospirillum thiophilum (97.5â%), Azospirillum rugosum (97.4â%) and Azospirillum zeae (97.2â%) and lower sequence similarity ( < 97.0â%) to all other species of the genus Azospirillum. According to DNA-DNA association, the relatedness values of strain CC-HIH038T with A. doebereinerae, A. thiophilum, A. rugosum and A. zeae were 51.8â%, 41.2â%, 56.5â% and 37.5â%, respectively. Strain CC-HIH038T was able to grow at 20-37 °C and pH 7.0-8.0. Strain CC-HIH038T gave positive amplification for dinitrogen reductase (nifH gene); the activity was recorded as 8.4ânmol ethylene h- 1. The predominant quinone system was ubiquinone Q-10 and the DNA G+C content was 68.8âmol%. The major fatty acids found in strain CC-HIH038T were C16â:â0, iso-C18â:â0, C16â:â0 3-OH, C14â:â0 3-OH/iso-C16â:â1 and C18â:â1ω7c/C18â:â1ω6c. Based on the distinct phylogenetic, phenotypic and chemotaxonomic traits together with results of comparative 16S rRNA gene sequence analysis, strain CC-HIH038T is considered to represent a novel species in the genus Azospirillum, for which the name Azospirillum agricola sp. nov. is proposed. The type strain is CC-HIH038T ( = BCRC 80909T = JCM 30827T).
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Phylogeny
/
Soil Microbiology
/
Azospirillum
/
Nitrogen Fixation
Country/Region as subject:
Asia
Language:
En
Journal:
Int J Syst Evol Microbiol
Journal subject:
MICROBIOLOGIA
Year:
2016
Document type:
Article
Country of publication:
United kingdom