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Molecular Study of the G1 Haplotypes of Echinococcus granulosus from Iran Based on Cytochrome C Oxidase (Subunit 1) Sequence.
Esmaelizad, Majid; Zeinedin, H; Razmaraii, Naser; Mirjalili, Ali.
Affiliation
  • Esmaelizad M; Department of Central Laboratory, Razi Vaccine and Serum Research Institute, Karaj, Iran. m.esmaelizad@rvsri.ac.ir.
Turkiye Parazitol Derg ; 39(4): 286-90, 2015 Dec.
Article in En | MEDLINE | ID: mdl-26809915
ABSTRACT

OBJECTIVE:

In this study, we attempted to identify new Echinococcus granulosus isolates in the North West provinces of Iran based on the mitochondrial cytochrome c oxidase subunit 1 (CO1) sequence.

METHODS:

Twenty-nine hydatid cysts from sheep and goats were collected. Genomic DNAs were extracted, and a partial sequence of the CO1 gene was amplified. Polymerase chain reaction products were cloned and sequenced with M13 primers in both directions.

RESULTS:

All Iranian isolates were located in G1 and G3 genotypes. For the first time, a new G1 haplotype in two Iranian isolates were identified.

CONCLUSION:

It seems that this new haplotype was transmitted from Jordan to Iran or vice versa.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Sheep Diseases / Goat Diseases / Cytochromes c1 / Electron Transport Complex IV / Echinococcus granulosus / Echinococcosis, Hepatic Limits: Animals Country/Region as subject: Asia Language: En Journal: Turkiye Parazitol Derg Year: 2015 Document type: Article Affiliation country: Iran

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Sheep Diseases / Goat Diseases / Cytochromes c1 / Electron Transport Complex IV / Echinococcus granulosus / Echinococcosis, Hepatic Limits: Animals Country/Region as subject: Asia Language: En Journal: Turkiye Parazitol Derg Year: 2015 Document type: Article Affiliation country: Iran