Detection of Free and Protein-Bound ortho-Quinones by Near-Infrared Fluorescence.
Anal Chem
; 88(4): 2399-405, 2016 Feb 16.
Article
in En
| MEDLINE
| ID: mdl-26813311
ABSTRACT
Aging and oxidative stress are two prominent pathological mechanisms for Parkinson's disease (PD) that are strongly associated with the degeneration of dopamine (DA) neurons in the midbrain. DA and other catechols readily oxidize into highly reactive o-quinone species that are precursors of neuromelanin (NM) pigment and under pathological conditions can modify and damage macromolecules. The role of DA oxidation in PD pathogenesis remains unclear in part due to the lack of appropriate disease models and the absence of a simple method for the quantification of DA-derived oxidants. Here, we describe a rapid, simple, and reproducible method for the quantification of o-quinones in cells and tissues that relies on the near-infrared fluorescent properties of these species. Importantly, we demonstrate that catechol-derived oxidants can be quantified in human neuroblastoma cells and midbrain dopamine neurons derived from induced pluripotent stem cells, providing a novel model to study the downstream actions of o-quinones. This method should facilitate further study of oxidative stress and DA oxidation in PD and related diseases that affect the dopaminergic system.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Quinones
/
Dopaminergic Neurons
/
Fluorescence
/
Infrared Rays
/
Neuroblastoma
Type of study:
Diagnostic_studies
Limits:
Humans
Language:
En
Journal:
Anal Chem
Year:
2016
Document type:
Article