Gametocyte clearance dynamics following oral artesunate treatment of uncomplicated falciparum malaria in Malian children.
Parasite
; 23: 3, 2016.
Article
in En
| MEDLINE
| ID: mdl-26839003
ABSTRACT
Artemisinin-based combination therapies decrease Plasmodium gametocyte carriage. However, the role of artesunate in monotherapy in vivo, the mechanisms involved, and the utility of gametocyte carriage as a potential tool for the surveillance of antimalarial resistance are poorly understood. In 2010-2011, we conducted an open-label, prospective efficacy study of artesunate as monotherapy in children 1-10 years of age with uncomplicated falciparum malaria in Bougoula-Hameau, Mali. Standard oral doses of artesunate were administered for 7 days and patients were followed up for 28 days. The data were compared to a similar study conducted in 2002-2004. Of 100 children enrolled in the 2010-2011 study, 92 were analyzed and compared to 217 children enrolled in the 2002-2004 study. The proportion of gametocyte carriers was unchanged at the end of treatment (23% at baseline vs. 24% on day 7, p = 1.0) and did not significantly decline until day 21 of follow-up (23% vs. 6%, p = 0.003). The mean gametocyte density at inclusion remained unchanged at the end of treatment (12 gametocytes/µL vs. 16 gametocytes/µL, p = 0.6). Overall, 46% of the 71 initial non-carriers had gametocytes detected by day 7. Similar results were found in the 2002-2004 study. In both studies, although gametocyte carriage significantly decreased by the end of the 28-day follow-up, artesunate did not clear mature gametocytes during treatment and did not prevent the appearance of new stage V gametocytes as assessed by light microscopy. Baseline gametocyte carriage was significantly higher 6 years after the deployment of artemisinin-based combination therapies in this setting.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Plasmodium falciparum
/
Carrier State
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Malaria, Falciparum
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Parasitemia
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Artemisinins
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Germ Cells
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Antimalarials
Type of study:
Clinical_trials
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Observational_studies
/
Risk_factors_studies
Limits:
Animals
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Child
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Child, preschool
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Female
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Humans
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Infant
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Male
Country/Region as subject:
Africa
Language:
En
Journal:
Parasite
Journal subject:
PARASITOLOGIA
Year:
2016
Document type:
Article
Affiliation country:
Mali