Increased ratio of ICOS(+) /PD-1(+) follicular helper T cells positively correlates with the development of human idiopathic membranous nephropathy.
Clin Exp Pharmacol Physiol
; 43(4): 410-6, 2016 Apr.
Article
in En
| MEDLINE
| ID: mdl-26845249
ABSTRACT
To identify the frequencies of different subsets of peripheral blood follicular helper T (Tfh) cells in human idiopathic membranous nephropathy (IMN), 39 patients with new onset IMN and 18 age- and gender-matched healthy controls (HC) were enrolled for this study. The frequency of Tfh cells in venous blood were measured by flow cytometry, while concentration of serum IL-21 was detected by enzyme-linked immunosorbent assay. Correlation between the clinical features of IMN and Tfh cells was assessed by Spearman's rank correlation test. Overall, the frequencies of total, ICOS(+) , and PD-1(+) Tfh cells were increased in IMN patients, while the ratio of ICOS(+) /PD-1(+) Tfh cells positively correlated with IMN progression. However, the elevated serum IL-21 level in three subgroups of IMN patients, stratified based on 24-h urine protein levels, was not statistically significant compared to HC. Nonetheless, intracellular IL-21 in Tfh cells was generally increased in all IMN patients, and closely correlated with IMN development. Finally, the frequency of IL-21(+) Tfh cells and the ratio of ICOS(+) /PD-1(+) Tfh cells were positively correlated with the estimated 24-h urine protein of IMN patients. The data indicated that Tfh cells contribute to the pathogenicity of IMN. The ratio of ICOS(+) /PD-1(+) Tfh cells and the frequency of IL-21(+) Tfh cells may be indicators for evaluating the IMN development.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Glomerulonephritis, Membranous
/
T-Lymphocytes, Helper-Inducer
/
Inducible T-Cell Co-Stimulator Protein
/
Programmed Cell Death 1 Receptor
Type of study:
Observational_studies
Limits:
Adult
/
Aged
/
Female
/
Humans
/
Male
/
Middle aged
Language:
En
Journal:
Clin Exp Pharmacol Physiol
Year:
2016
Document type:
Article
Affiliation country:
China