Production and evaluation of recombinant hirudin.
Semin Thromb Hemost
; 15(3): 288-92, 1989 Jul.
Article
in En
| MEDLINE
| ID: mdl-2688101
ABSTRACT
Hirudin, a 65 amino acid polypeptide form the medicinal leech, is an extremely efficient and specific thrombin inhibitor whose therapeutic potential has been demonstrated in a number of animal models. We have developed protocols for the production of recombinant hirudin by secretion from S. cerevisiae and carried out a full biologic evaluation of the purified product. These studies showed that natural and recombinant hirudin was similar in structure and in biologic function in vitro. Moreover, the recombinant protein displayed strong antithrombotic activity in several experimental thrombosis models in vivo, confirming the molecule's promise in the therapy of thrombotic disorders.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Recombinant Proteins
/
Hirudins
Type of study:
Guideline
Limits:
Animals
Language:
En
Journal:
Semin Thromb Hemost
Year:
1989
Document type:
Article
Affiliation country:
France