The MAP kinase pathway coordinates crossover designation with disassembly of synaptonemal complex proteins during meiosis.
Elife
; 5: e12039, 2016 Feb 27.
Article
in En
| MEDLINE
| ID: mdl-26920220
Asymmetric disassembly of the synaptonemal complex (SC) is crucial for proper meiotic chromosome segregation. However, the signaling mechanisms that directly regulate this process are poorly understood. Here we show that the mammalian Rho GEF homolog, ECT-2, functions through the conserved RAS/ERK MAP kinase signaling pathway in the C. elegans germline to regulate the disassembly of SC proteins. We find that SYP-2, a SC central region component, is a potential target for MPK-1-mediated phosphorylation and that constitutively phosphorylated SYP-2 impairs the disassembly of SC proteins from chromosomal domains referred to as the long arms of the bivalents. Inactivation of MAP kinase at late pachytene is critical for timely disassembly of the SC proteins from the long arms, and is dependent on the crossover (CO) promoting factors ZHP-3/RNF212/Zip3 and COSA-1/CNTD1. We propose that the conserved MAP kinase pathway coordinates CO designation with the disassembly of SC proteins to ensure accurate chromosome segregation.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Synaptonemal Complex
/
Protein Processing, Post-Translational
/
Chromosome Segregation
/
MAP Kinase Signaling System
/
Guanine Nucleotide Exchange Factors
/
Crossing Over, Genetic
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Caenorhabditis elegans Proteins
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Meiosis
Limits:
Animals
Language:
En
Journal:
Elife
Year:
2016
Document type:
Article
Affiliation country:
United States
Country of publication:
United kingdom