Bioorthogonal Copper Free Click Chemistry for Labeling and Tracking of Chondrocytes In Vivo.
Bioconjug Chem
; 27(4): 927-36, 2016 Apr 20.
Article
in En
| MEDLINE
| ID: mdl-26930274
ABSTRACT
Establishment of an appropriate cell labeling and tracking method is essential for the development of cell-based therapeutic strategies. Here, we are introducing a new method for cell labeling and tracking by combining metabolic gylcoengineering and bioorthogonal copper-free Click chemistry. First, chondrocytes were treated with tetraacetylated N-azidoacetyl-D-mannosamine (Ac4ManNAz) to generate unnatural azide groups (-N3) on the surface of the cells. Subsequently, the unnatural azide groups on the cell surface were specifically conjugated with near-infrared fluorescent (NIRF) dye-tagged dibenzyl cyclooctyne (DBCO-650) through bioorthogonal copper-free Click chemistry. Importantly, DBCO-650-labeled chondrocytes presented strong NIRF signals with relatively low cytotoxicity and the amounts of azide groups and DBCO-650 could be easily controlled by feeding different amounts of Ac4ManNAz and DBCO-650 to the cell culture system. For the in vivo cell tracking, DBCO-650-labeled chondrocytes (1 × 10(6) cells) seeded on the 3D scaffold were subcutaneously implanted into mice and the transplanted DBCO-650-labeled chondrocytes could be effectively tracked in the prolonged time period of 4 weeks using NIRF imaging technology. Furthermore, this new cell labeling and tracking technology had minimal effect on cartilage formation in vivo.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Cartilage
/
Copper
/
Chondrocytes
/
Click Chemistry
Limits:
Animals
Language:
En
Journal:
Bioconjug Chem
Journal subject:
BIOQUIMICA
Year:
2016
Document type:
Article