Isolation of an electrogenic K+/Ca2+ ionophore from an ionophoroprotein of beef heart mitochondria.
Proc Natl Acad Sci U S A
; 74(9): 3667-71, 1977 Sep.
Article
in En
| MEDLINE
| ID: mdl-269422
ABSTRACT
A K+/Ca2+ electrogenic ionophore has been isolated from an ionophoroprotein of beef heart mitochondria and identified as a neutral peptide of molecular weight 1600. The amino acid composition and cationic specificity of the ionophore have been determined. The free ionophore was released from the ionophoroprotein as a consequence of tryptic digestion. The ionophoroprotein can be converted to an ionophoro peptide (molecular weight 5,100) by proteolysis without release of the free ionophore. The isolation of a K+/Ca2+ ionophore thus provides an introduction to the general technology of extracting ionophoro proteins and ofreleasing ionophores from these proteins by proteolytic digestion.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Potassium
/
Calcium
/
Ionophores
/
Mitochondria, Heart
/
Muscle Proteins
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Proc Natl Acad Sci U S A
Year:
1977
Document type:
Article
Country of publication:
EEUU
/
ESTADOS UNIDOS
/
ESTADOS UNIDOS DA AMERICA
/
EUA
/
UNITED STATES
/
UNITED STATES OF AMERICA
/
US
/
USA