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Overcoming heterologous protein interdependency to optimize P450-mediated Taxol precursor synthesis in Escherichia coli.
Biggs, Bradley Walters; Lim, Chin Giaw; Sagliani, Kristen; Shankar, Smriti; Stephanopoulos, Gregory; De Mey, Marjan; Ajikumar, Parayil Kumaran.
Affiliation
  • Biggs BW; Manus Biosynthesis, Cambridge, MA 02138; Masters in Biotechnology Program, Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL 60208;
  • Lim CG; Manus Biosynthesis, Cambridge, MA 02138;
  • Sagliani K; Manus Biosynthesis, Cambridge, MA 02138;
  • Shankar S; Manus Biosynthesis, Cambridge, MA 02138;
  • Stephanopoulos G; Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139;
  • De Mey M; Manus Biosynthesis, Cambridge, MA 02138; Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139; Laboratory for Industrial Biotechnology and Biocatalysis, Ghent University, Coupure Links 653, B-9000, Belgium marjan.demey@ugent.be pkaji@manusbio.com.
  • Ajikumar PK; Manus Biosynthesis, Cambridge, MA 02138; marjan.demey@ugent.be pkaji@manusbio.com.
Proc Natl Acad Sci U S A ; 113(12): 3209-14, 2016 Mar 22.
Article in En | MEDLINE | ID: mdl-26951651
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Paclitaxel / Cytochrome P-450 Enzyme System / Escherichia coli / Antineoplastic Agents, Phytogenic Language: En Journal: Proc Natl Acad Sci U S A Year: 2016 Document type: Article Country of publication: United States
Fulltext
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Paclitaxel / Cytochrome P-450 Enzyme System / Escherichia coli / Antineoplastic Agents, Phytogenic Language: En Journal: Proc Natl Acad Sci U S A Year: 2016 Document type: Article Country of publication: United States