Generation and Validation of Monoclonal Antibodies Against the Maltose Binding Protein.
Monoclon Antib Immunodiagn Immunother
; 35(2): 104-8, 2016 Apr.
Article
in En
| MEDLINE
| ID: mdl-26982821
ABSTRACT
The maltose binding protein (MBP) is a commonly used protein tag. Two monoclonal antibodies (mAbs) were generated against the MBP by immunizing mice with purified 6xHis-tagged MBP (6xHis-MBP). A nontoxic adjuvant cocktail of poly(IC) and anti-CD40 mAb was used. The two mAbs, 3D7 and 2A1, are demonstrated to be effective in immunoprecipitation, immunoblotting, western blot hybridization, and the ELISA assay. These two mAbs are available individually or in combination at cost through the Developmental Studies Hybridoma Bank, a nonprofit National Resource created by the National Institutes of Health.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Enzyme-Linked Immunosorbent Assay
/
Maltose-Binding Proteins
/
Antibodies, Monoclonal
Limits:
Animals
/
Humans
Language:
En
Journal:
Monoclon Antib Immunodiagn Immunother
Year:
2016
Document type:
Article