Development of an angiogenesis animal model featuring brain arteriovenous malformation histological characteristics.
J Neurointerv Surg
; 9(2): 204-210, 2017 Feb.
Article
in En
| MEDLINE
| ID: mdl-27013231
ABSTRACT
BACKGROUND:
Angiogenesis has a key role in the formation and evolution of brain arteriovenous malformations (AVMs). Numerous models have been developed aiming to recreate configuration of brain AVMs.OBJECTIVE:
To develop an animal model sharing the same pathological characteristics as human brain AVMs. MATERIALS ANDMETHODS:
Ten pigs were divided into two groups. Five animals underwent endovascular left common carotid artery (CCA) and external carotid artery (ECA) occlusion and five animals served as controls. DSA, associated with 3D-rotational angiography, was performed at day 0 and at 3â months in both groups. The volume of the retia was calculated. Vascular endothelial growth factor (VEGF)-A serum levels were measured in both groups at the same time intervals. Finally, the animals were sacrificed at 3â months and the retia were harvested for pathological and immunohistochemistry examinations.RESULTS:
At 3â months, a significantly higher rete volume was seen in group A than in group B (2.92±0.33â mL vs 1.87±0.69â mL, respectively; p=0.016). There was a trend for increased VEGF-A levels in group A at 3â months. In the occlusion group, histological findings showed significant reduction of media thickness and disrupted internal elastic lamina; immunohistochemistry findings showed strong reactivity for VEGF receptors and interleukin 6.CONCLUSIONS:
Unilateral endovascular occlusion of the CCA-ECA results in angiogenesis triggering of the rete mirabile with both significant augmentation of the rete volume and histological evidence of pro-angiogenic stimulation.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Intracranial Arteriovenous Malformations
/
Neovascularization, Pathologic
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
J Neurointerv Surg
Year:
2017
Document type:
Article
Affiliation country:
France