Adaptation and Validation of E-Probe Diagnostic Nucleic Acid Analysis for Detection of Escherichia coli O157:H7 in Metagenomic Data from Complex Food Matrices.

Blagden, Trenna; Schneider, William; Melcher, Ulrich; Daniels, Jon; Fletcher, Jacqueline

Blagden, Trenna; Schneider, William; Melcher, Ulrich; Daniels, Jon; Fletcher, Jacqueline.

Affiliation

Blagden T; Department of Entomology and Plant Pathology, 127 NRC, Oklahoma State University, Stillwater, Oklahoma 74078, USA. trenna.bladgen@okstate.edu.
Schneider W; U.S. Department of Agriculture, Agricultural Research Service, Room 139, Building 1301, 1301 Ditto Avenue, Fort Detrick, Maryland 21702, USA.
Melcher U; Department of Biochemistry and Molecular Biology, 246 NRC, Oklahoma State University, Stillwater, Oklahoma 74078, USA.
Daniels J; Department of Entomology and Plant Pathology, 127 NRC, Oklahoma State University, Stillwater, Oklahoma 74078, USA.
Fletcher J; Department of Entomology and Plant Pathology, 127 NRC, Oklahoma State University, Stillwater, Oklahoma 74078, USA.

J Food Prot ; 79(4): 574-81, 2016 Apr.

Article in En | MEDLINE | ID: mdl-27052861

Subject(s)

DNA, Bacterial/genetics; Escherichia coli O157/genetics; Foodborne Diseases/microbiology; Medicago sativa/microbiology; Metagenomics/methods; Vegetables/microbiology; Escherichia coli O157/classification; Escherichia coli O157/isolation & purification; Food Contamination/analysis; High-Throughput Nucleotide Sequencing; Humans; United States

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Vegetables / DNA, Bacterial / Escherichia coli O157 / Medicago sativa / Metagenomics / Foodborne Diseases Type of study: Diagnostic_studies Limits: Humans Country/Region as subject: America do norte Language: En Journal: J Food Prot Year: 2016 Document type: Article Affiliation country: United States Country of publication: United States

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