Quantitative Single-Cell mRNA Analysis in Hydrogel Beads.
Angew Chem Int Ed Engl
; 55(23): 6698-701, 2016 06 01.
Article
in En
| MEDLINE
| ID: mdl-27075637
ABSTRACT
In recent years, technologies capable of analyzing single cells have emerged that are transforming many fields of biological research. Herein we report how DNA-functionalized hydrogel beads can serve as a matrix to capture mRNA from lysed single cells. mRNA quantification free of pre-amplification bias is ensured by using padlock probes and rolling circle amplification followed by hybridization with fluorescent probes. The number of transcripts in individual cells is assessed by simply counting fluorescent dots inside gel beads. The method extends the potential of existing techniques and provides a general platform for capturing molecules of interest from single cells.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
RNA, Messenger
/
Hydrogels
/
Nucleic Acid Amplification Techniques
Limits:
Humans
Language:
En
Journal:
Angew Chem Int Ed Engl
Year:
2016
Document type:
Article
Affiliation country:
Netherlands