In vitro expression and analysis of the 826 human G protein-coupled receptors.
Protein Cell
; 7(5): 325-37, 2016 05.
Article
in En
| MEDLINE
| ID: mdl-27085723
ABSTRACT
G protein-coupled receptors (GPCRs) are involved in all human physiological systems where they are responsible for transducing extracellular signals into cells. GPCRs signal in response to a diverse array of stimuli including light, hormones, and lipids, where these signals affect downstream cascades to impact both health and disease states. Yet, despite their importance as therapeutic targets, detailed molecular structures of only 30 GPCRs have been determined to date. A key challenge to their structure determination is adequate protein expression. Here we report the quantification of protein expression in an insect cell expression system for all 826 human GPCRs using two different fusion constructs. Expression characteristics are analyzed in aggregate and among each of the five distinct subfamilies. These data can be used to identify trends related to GPCR expression between different fusion constructs and between different GPCR families, and to prioritize lead candidates for future structure determination feasibility.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Receptors, G-Protein-Coupled
Type of study:
Prognostic_studies
Limits:
Animals
/
Humans
Language:
En
Journal:
Protein Cell
Journal subject:
BIOQUIMICA
Year:
2016
Document type:
Article
Affiliation country:
China