Efferocytosis as a regulator of macrophage chemokine receptor expression and polarization.
Eur J Immunol
; 46(7): 1592-9, 2016 07.
Article
in En
| MEDLINE
| ID: mdl-27139187
ABSTRACT
Efferocytosis has been suggested to promote macrophage resolution programs that are dependent on motility and emigration, however, few studies have addressed directed migration in resolving macrophages. In this report, we hypothesized that efferocytosis would induce differential chemokine receptor expression. Polarized macrophage populations, including macrophages actively engaged in efferocytosis, were characterized by PCR array and traditional transwell motility assays. We identified specific up-regulation of chemokine receptor CXCR4 on both mouse and human macrophages and characterized in vivo expression of CXCR4 in a resolving model of murine peritonitis. Using adoptive transfer and AMD3100 blocking, we confirmed a role for CXCR4 in macrophage egress to draining lymphatics. Collectively these data provide an important mechanistic link between efferocytosis and macrophage emigration.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Phagocytosis
/
Gene Expression Regulation
/
Receptors, Chemokine
/
Immunomodulation
/
Macrophage Activation
/
Macrophages
Limits:
Animals
Language:
En
Journal:
Eur J Immunol
Year:
2016
Document type:
Article
Affiliation country:
United States