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Transfection of Human Keratinocytes with Nucleoside-Modified mRNA Encoding CPD-Photolyase to Repair DNA Damage.
Boros, Gábor; Karikó, Katalin; Muramatsu, Hiromi; Miko, Edit; Emri, Eszter; Hegedus, Csaba; Emri, Gabriella; Remenyik, Éva.
Affiliation
  • Boros G; Department of Dermatology, Faculty of Medicine, University of Debrecen, Nagyerdei krt. 98, Debrecen, 4032, Hungary.
  • Karikó K; Department of Neurosurgery, University of Pennsylvania, 3450 Hamilton Walk, Philadelphia, PA, 19104, USA.
  • Muramatsu H; BioNTech RNA Pharmaceuticals, An der Goldgrube 12, Mainz, 55131, Germany.
  • Miko E; Department of Neurosurgery, University of Pennsylvania, 3450 Hamilton Walk, Philadelphia, PA, 19104, USA.
  • Emri E; BioNTech RNA Pharmaceuticals, An der Goldgrube 12, Mainz, 55131, Germany.
  • Hegedus C; Department of Dermatology, Faculty of Medicine, University of Debrecen, Nagyerdei krt. 98, Debrecen, 4032, Hungary.
  • Emri G; MTA-DE Lendület Laboratory of Cellular Metabolism Research Group of the Hungarian Academy of Sciences, University of Debrecen, Nagyerdei krt. 98, Debrecen, 4032, Hungary.
  • Remenyik É; Department of Dermatology, Faculty of Medicine, University of Debrecen, Nagyerdei krt. 98, Debrecen, 4032, Hungary.
Methods Mol Biol ; 1428: 219-28, 2016.
Article in En | MEDLINE | ID: mdl-27236802
ABSTRACT
In vitro-synthesized mRNA containing nucleoside modifications has great therapeutical potential to transiently express proteins with physiological importance. One such protein is photolyase which rapidly removes UV-induced DNA damages, but this enzyme is absent in humans. Here, we apply a novel mRNA-based platform to achieve functional nonhuman photolyase production in cultured human keratinocytes. Transfection of nucleoside-modified mRNA encoding photolyase leads to accelerated repair of DNA photolesions in human keratinocytes.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: RNA, Messenger / Keratinocytes / Deoxyribodipyrimidine Photo-Lyase / Nucleosides Limits: Humans Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2016 Document type: Article Affiliation country: Hungary
Fulltext
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: RNA, Messenger / Keratinocytes / Deoxyribodipyrimidine Photo-Lyase / Nucleosides Limits: Humans Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2016 Document type: Article Affiliation country: Hungary