Cell spreading and viability on zein films may be facilitated by transglutaminase.
Colloids Surf B Biointerfaces
; 145: 839-844, 2016 Sep 01.
Article
in En
| MEDLINE
| ID: mdl-27315332
ABSTRACT
Zein is a biocompatible corn protein potentially useful in the development of biomaterials. In this study, the deposition of zein on oxygen plasma treated glass cover slips significantly enhanced cell spreading and viability. The mechanism for cellular response to zein coated surfaces was thought to involve the polyglutamine peptides on the zein structure. We hypothesized that zein was a substrate for tissue transglutaminase (tTG), an extracellular enzyme involved in cell-surface interactions. SDS-PAGE results suggested an interaction between zein and tTG, where zein was the glutamine donor. Cross-linking between zein and tTG may be the first step in successful cell adhesion and spreading.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Zein
/
Membranes, Artificial
Limits:
Animals
Language:
En
Journal:
Colloids Surf B Biointerfaces
Journal subject:
QUIMICA
Year:
2016
Document type:
Article
Affiliation country:
United States