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Proteomic analyses of brain tumor cell lines amidst the unfolded protein response.
Redzic, Jasmina S; Gomez, Joe D; Hellwinkel, Justin E; Anchordoquy, Thomas J; Graner, Michael W.
Affiliation
  • Redzic JS; Skaggs School of Pharmacy and Pharmaceutical Sciences, University of Colorado Denver Anschutz Medical Campus, Aurora, CO, USA.
  • Gomez JD; Skaggs School of Pharmacy and Pharmaceutical Sciences, University of Colorado Denver Anschutz Medical Campus, Aurora, CO, USA.
  • Hellwinkel JE; Department of Neurosurgery, University of Colorado Denver Anschutz Medical Campus, Aurora, CO, USA.
  • Anchordoquy TJ; Skaggs School of Pharmacy and Pharmaceutical Sciences, University of Colorado Denver Anschutz Medical Campus, Aurora, CO, USA.
  • Graner MW; Department of Neurosurgery, University of Colorado Denver Anschutz Medical Campus, Aurora, CO, USA.
Oncotarget ; 7(30): 47831-47847, 2016 Jul 26.
Article in En | MEDLINE | ID: mdl-27323862
ABSTRACT
Brain tumors such as high grade gliomas are among the deadliest forms of human cancers. The tumor environment is subject to a number of cellular stressors such as hypoxia and glucose deprivation. The persistence of the stressors activates the unfolded proteins response (UPR) and results in global alterations in transcriptional and translational activity of the cell. Although the UPR is known to effect tumorigenesis in some epithelial cancers, relatively little is known about the role of the UPR in brain tumors. Here, we evaluated the changes at the molecular level under homeostatic and stress conditions in two glioma cell lines of differing tumor grade. Using mass spectrometry analysis, we identified proteins unique to each condition (unstressed/stressed) and within each cell line (U87MG and UPN933). Comparing the two, we find differences between both the conditions and cell lines indicating a unique profile for each. Finally, we used our proteomic data to identify the predominant pathways within these cells under unstressed and stressed conditions. Numerous predominant pathways are the same in both cell lines, but there are differences in biological and molecular classifications of the identified proteins, including signaling mechanisms, following UPR induction; we see that relatively minimal proteomic alterations can lead to signaling changes that ultimately promote cell survival.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Brain Neoplasms / Glioma / Neoplasm Proteins Limits: Humans Language: En Journal: Oncotarget Year: 2016 Document type: Article Affiliation country: United States

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Brain Neoplasms / Glioma / Neoplasm Proteins Limits: Humans Language: En Journal: Oncotarget Year: 2016 Document type: Article Affiliation country: United States