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Data on the characterization of follicle-stimulating hormone monoclonal antibodies and localization in Japanese eel pituitary.
Kim, Dae-Jung; Park, Chae-Won; Byambaragchaa, Munkhzaya; Kim, Shin-Kwon; Lee, Bae-Ik; Hwang, Hyung-Kyu; Myeong, Jeong-In; Hong, Sun-Mee; Kang, Myung-Hwa; Min, Kwan-Sik.
Affiliation
  • Kim DJ; Aquaculture Research Division, National Institute of Fisheries Science (NIFS), Busan 46083, Republic of Korea.
  • Park CW; Animal Biotechnology, Graduate School of Future Convergence Technology, Institute of Genetic Engineering, Hankyong National University, Anseong 17579, Republic of Korea.
  • Byambaragchaa M; Animal Biotechnology, Graduate School of Future Convergence Technology, Institute of Genetic Engineering, Hankyong National University, Anseong 17579, Republic of Korea.
  • Kim SK; Aquaculture Research Division, National Institute of Fisheries Science (NIFS), Busan 46083, Republic of Korea.
  • Lee BI; Aquaculture Research Division, National Institute of Fisheries Science (NIFS), Busan 46083, Republic of Korea.
  • Hwang HK; Aquaculture Research Division, National Institute of Fisheries Science (NIFS), Busan 46083, Republic of Korea.
  • Myeong JI; Aquaculture Research Division, National Institute of Fisheries Science (NIFS), Busan 46083, Republic of Korea.
  • Hong SM; Dept. of Research and Development, Institute of Gyeongbuk Marine Bioindustry, Ulgin, 36315, Republic of Korea.
  • Kang MH; Department of Food Science & Nutrition, Hoseo University, Asan 31499, Republic of Korea.
  • Min KS; Animal Biotechnology, Graduate School of Future Convergence Technology, Institute of Genetic Engineering, Hankyong National University, Anseong 17579, Republic of Korea.
Data Brief ; 8: 404-10, 2016 Sep.
Article in En | MEDLINE | ID: mdl-27331121
ABSTRACT
Monoclonal antibodies were generated against recombinant follicle-stimulating hormone (rec-FSH) from Japanese eel Anguilla japonica; rec-FSH was produced in Escherichia coli and purified using Ni-NTA Sepharose column chromatography. In support of our recent publication, "Production and characterization of monoclonal antibodies against recombinant tethered follicle-stimulating hormone from Japanese eel Anguilla japonica" [1], it was important to characterize the specificity of eel follicle-stimulating hormone antibodies. Here, the production and ELISA system of these monoclonal antibodies are presented. The affinity-purified monoclonal antibodies specifically detected eel rec-FSH in ELISA and on western blots of rec-FSH produced from CHO cells. Immunohistochemical analysis revealed that FSH staining was specifically localized in the eel pituitary.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: Data Brief Year: 2016 Document type: Article
Fulltext
Add to My VHL
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Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: Data Brief Year: 2016 Document type: Article